Journal: Cancers

Article Title: TP-0903 Is Active in Preclinical Models of Acute Myeloid Leukemia with TP53 Mutation/Deletion.

doi: 10.3390/cancers15010029

Figure Lengend Snippet: Figure 3. TP-0903 induces DNA damage response in TP53 mutant AML cell lines. (A) MV4-11 (R248W), (B) HL-60, and (C) Kasumi-1 cells were treated with DMSO or TP-0903 at indicated concentrations for 4 h. Immunoblotting was performed to determine the expression of pChk1 (Ser345), pChk2 (Thr68), and pH2AX (Ser139/Tyr142). Vinculin served as the loading control for pChk1/Chk1 and pChk2/Chk2 blots. GAPDH served as the loading control for pH2AX/H2AX blots. Immunoblots were quantified against respective loading controls (vinculin or GAPDH) using ImageJ. Blots are representative of 2–3 independent experiments.

Article Snippet: Antibodies against p53 (48818; clone DO-7), p21 (2947; clone 12D1), pH2AX (Ser 139) (80312; clone D7T2V), pH2AX (Ser139/Tyr142) (5438; clone N/A), H2AX (7631; clone D17A3), pAURKA(Thr288)/B(Thr232)/C(Thr198) (2914; clone D13A11), AURKA (91590; clone D3V7T), AURKB (3094; clone N/A), pChk1 (Ser345) (2348; clone 133D3), pChk2 (Thr68) (2197; clone C13C1), Chk1 (2360; clone 2G1D5), Chk2 (6334; clone D9C6), vinculin (13901; clone E1E9V), GAPDH (5174S; clone D16H11), and HRP-conjugate secondary antirabbit (7074) were obtained from Cell Signaling Technology (CST) (Danvers, MA, USA).

Techniques: Mutagenesis, Western Blot, Expressing, Control

Journal: Journal of Ayurveda and Integrative Medicine

Article Title: A herbal formulation inhibits growth and survival of lung cancer cells through DNA damage and apoptosis - in vitro and in vivo studies

doi: 10.1016/j.jaim.2025.101313

Figure Lengend Snippet: Effect of SL on DNA damage and response (DDR) proteins in A549 cells. A549 cells were treated with SL for 48 h and stained with acridine orange/ethidium bromide (AO/EB) and observed under the fluorescent microscope. (A) Images of A549 stained with AO/ET at 200x magnification. (B) Percentage of apoptotic cells. (C) SL-induced DNA fragmentation in A549 cells. (D) SL affected DNA damage response and repair-related proteins namely, Chk1, Chk2, and Rad51 in A549 cells. Data points are the means ± s.e.m. of three independent experiments. Bars, s.e.m, ∗∗∗P < 0.001. SL, Safoof Lajward . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibodies for Bcl-2, #2872; Bax, #2772; Chk1, #2360; Chk2, #3440; Rad51, #82263; PCNA, #2586; Ki-67, #9449; Cleaved caspase3, # 9664; Cleaved PARP, #5625; and Beta-actin, #A3853, and secondary antibodies, anti-mouse, #7076 and anti-rabbit, #7074P2, were acquired from Cell Signaling Technology (CST), USA.

Techniques: Staining, Microscopy

Journal: Journal of Ayurveda and Integrative Medicine

Article Title: A herbal formulation inhibits growth and survival of lung cancer cells through DNA damage and apoptosis - in vitro and in vivo studies

doi: 10.1016/j.jaim.2025.101313

Figure Lengend Snippet: Effect of SL on molecular markers of proliferation, apoptosis, and DDR proteins in tumor tissue . (A) H&E and immunohistochemical (IHC) profiling of A549 tumor tissues from nude mice showing decreased proliferation, Ki-67 positivity, and increased apoptosis, cleaved caspase 3, and cleaved PARP positivity in the SL treated group. H&E images at 200x and IHC images at 400x magnification, scale bar is 50 μm. (B–D) Quantification of Ki-67, cleaved caspase 3, and cleaved PARP positive cells. (E) Western bot analysis of SL treated tumors on apoptosis, DNA damage pathway, and cell survival proteins (PCNA, Bax, Bcl-2, Chk1, Chk2, and Rad51) in xenograft tumor tissues. (F–J) Quantification of a Western blot for the protein expression levels of PCNA, Chk1, Chk2, Rad51, and Bax/Bcl-2 ratio. T1, T2, and T3 are different tumor samples from the control and treated groups. ∗P < 0.05, ∗∗∗P < 0.001. SL, Safoof Lajward .

Article Snippet: Primary antibodies for Bcl-2, #2872; Bax, #2772; Chk1, #2360; Chk2, #3440; Rad51, #82263; PCNA, #2586; Ki-67, #9449; Cleaved caspase3, # 9664; Cleaved PARP, #5625; and Beta-actin, #A3853, and secondary antibodies, anti-mouse, #7076 and anti-rabbit, #7074P2, were acquired from Cell Signaling Technology (CST), USA.

Techniques: Immunohistochemical staining, Western Blot, Expressing, Control

Journal: PLoS ONE

Article Title: Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model

doi: 10.1371/journal.pone.0176747

Figure Lengend Snippet: A . Quantification of CellTiterBlue (CTB) viability assay in human A549 and H441 NSCLC cell lines treated with vehicle, STA-8666 and irinotecan in doses of 0.1, 1, 10 μM for up to 5 days. Chart curves represent average data from two independent runs performed with duplicate samples. B-E . Western blot analysis of phosphorylated (p) and total (t) ERK1/2 ( B ), AKT ( C ), CHK2 ( D ) and CDK1 ( E ) protein expression in human A549 and H441 NSCLC cell lines, at 48 hours after treatment with vehicle (V), STA-8666 and irinotecan in doses of 0.1, 1, or 10 μM, as indicated. Histograms represent data from two independent experiments. All graphs: *, p≤0.05; **, p≤0.01; ***, p≤0.001 relative to vehicle controls.

Article Snippet: Primary antibodies were used at a 1:1000 dilution and included: anti-PARP (#9542L Cell Signaling, Beverly, MA), anti-pT 202 /Y 204 -ERK1/2 (#4996S Cell Signaling, Beverly, MA), anti-Erk1/2, (#4696S Cell Signaling, Beverly, MA), anti-p T308 Akt, (#2965S Cell Signaling, Beverly, MA), anti-Akt, (#2920S Cell Signaling, Beverly, MA), anti-p T68 Chk2, (#MAB1626 R&D Systems, Minneapolis, MN); anti-Chk2, (#3440 Cell Signaling, Beverly, MA), anti-p Y15 CDK1, (#9111L Cell Signaling, Beverly, MA), anti-CDK1, (#SC54 Santa Cruz Biotechnology, Dallas, TX); anti-E-cadherin, (#3195S Cell Signaling, Beverly, MA); anti-vimentin, (ab92547, Abcam, Cambridge, MA), anti-Snail, (ab180714, Abcam, Cambridge, MA), and anti-vinculin (mouse, monoclonal hVIN-1, #V9131, Sigma-Aldrich, St. Louis, MO).

Techniques: Viability Assay, Western Blot, Expressing

Journal: International Journal of Medical Sciences

Article Title: Cedrus atlantica Extract Suppress Glioblastoma Growth through Promotion of Genotoxicity and Apoptosis: In Vitro and In Vivo Studies

doi: 10.7150/ijms.54468

Figure Lengend Snippet: Primary antibodies information.

Article Snippet: p-Chk2 , Biorbyt Ltd. , Orb5862 , Rabbit , WB: 1/1000.

Techniques: